Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/3970
Title: Crystal-structure of the Complex Between Human Carbonic Anhydrase-ii and the Aromatic Inhibitor 1,2,4-triazole
Authors: Mangani, Stefano 
A., Liljas
Keywords: human carbonic anhydrase; crystal structure; mechanism; inhibitor
Issue Date: 1993
Project: None 
Journal: JOURNAL OF MOLECULAR BIOLOGY
Abstract: 
The X-ray crystal structure of the complex between human carbonic anhydrase II and the inhibitor 1,2,4-triazole has been refined at 1·9 Å resolution to a final R-factor of 0·153. Triazole is an analogue of the competitive inhibitor imidazole, but the crystal structure shows a different type of binding to the enzyme, 1,2,4-Triazole is directly bound to the zinc(II) ion through the nitrogen in position 4, replacing the native water/hydroxyl (Wat263) in a distorted four-co-ordinated complex. The interaction of the inhibitor with the active site is completed by two hydrogen bonds to Oγ of Thr200 and to the amide nitrogen atom of Thr199 through the two adjacent N-1 and N-2 atoms. The binding site of triazole overlaps the proposed binding sites for the substrates, explaining the observed competitive behaviour of the inhibitor towards CO2/HCO-3 under equilibrium conditions.
Description: 
47990
URI: http://hdl.handle.net/20.500.12779/3970
ISSN: 0022-2836
DOI: 10.1006/jmbi.1993.1365
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.