Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/3915
Title: X-ray-diffraction Study of the Interaction Between Carboxypeptidase-a and (s)-(+)-1-amino-2-phenylethyl Phosphonic Acid
Authors: Mangani, Stefano 
P., Carloni
P., Orioli
Keywords: x-ray; crystal structure; carboxypeptidase A; inhibitor
Issue Date: 1992
Project: None 
Journal: EUROPEAN JOURNAL OF BIOCHEMISTRY
Abstract: 
The structure of the carboxypeptidase A complex with the inhibitor (S)-(+)-1-amino-2-phenylethylphosphonic acid has been determined at 0.23 nm resolution. The DELTA-F map shows electron-density peaks both in the S1 and S1' sites, where the inhibitor molecule can be modeled in two different orientations with approximate 50% occupancy. In the proposed model, the phosphonate group binds to the zinc ion in a monodentate fashion. Other anchoring groups for the inhibitor molecule are Arg127 (hydrogen bonds with the phosphonate oxygen atoms) and Glu270 (hydrogen bond with the amino group in one of the two orientations). A recent spectroscopic investigation of the complex between cobalt(II) carboxypeptidase A and (S)-(+)-1-amino-2-phenylethylphosphonic acid is essentially in agreement with our results.
Description: 
47997
URI: http://hdl.handle.net/20.500.12779/3915
ISSN: 0014-2956
DOI: 10.1111/j.1432-1033.1992.tb19843.x
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