Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/3861
Title: The Dependence of Cis-diamminedichloroplatinum(ii) Binding To Dna Upon the Gc Content - A Thermal and Spectrophotometric Investigation
Authors: S. G., Haroutiunian
B., Bruni
R., Monnanni
P., Orioli
Mangani, Stefano 
Keywords: DNA; calorimetry
Issue Date: 1991
Project: None 
Journal: INORGANICA CHIMICA ACTA
Abstract: 
The binding of cis-diamminedichloroplatinum(II) (cis-DDP) to DNA is investigated as a function of the GC content and of the cis-DDP concentration. Analysis of the melting curves and of the UV and CD spectra shows that binding of cis-DDP to DNA is highly sequence specific, GC rich DNAs being destabilized more effectively. At low levels of platination, stabilization of GC- and destabilization of AT-base pairs can be noted. In AT rich DNA, cis-DDP binding favours the B --> A conformational transition. It is also shown that, in addition to specific interactions between cis-DDP and DNA, several non-specific processes take place.
Description: 
47999
URI: http://hdl.handle.net/20.500.12779/3861
ISSN: 0020-1693
DOI: 10.1016/S0020-1693(00)83054-6
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