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|Title:||The Dependence of Cis-diamminedichloroplatinum(ii) Binding To Dna Upon the Gc Content - A Thermal and Spectrophotometric Investigation||Authors:||S. G., Haroutiunian
|Keywords:||DNA; calorimetry||Issue Date:||1991||Project:||None||Journal:||INORGANICA CHIMICA ACTA||Abstract:||
The binding of cis-diamminedichloroplatinum(II) (cis-DDP) to DNA is investigated as a function of the GC content and of the cis-DDP concentration. Analysis of the melting curves and of the UV and CD spectra shows that binding of cis-DDP to DNA is highly sequence specific, GC rich DNAs being destabilized more effectively. At low levels of platination, stabilization of GC- and destabilization of AT-base pairs can be noted. In AT rich DNA, cis-DDP binding favours the B --> A conformational transition. It is also shown that, in addition to specific interactions between cis-DDP and DNA, several non-specific processes take place.
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