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|Title:||Single step purification of a synthetic peptide fragment of neurofilaments by preparative high performance liquid chromatography||Authors:||Rustici, M
|Issue Date:||1989||Project:||None||Journal:||JOURNAL OF LIQUID CHROMATOGRAPHY||Abstract:||
It was recently shown that fragment Lys-Ser-Pro-Val (1,2) is the major site in vivo neurofilament phosforilation. A suitable method for the preparation of this peptide was therefore studied paying particular attention to the purification step. Even thought solubility of this peptide in methyl t-butil ether does not allow the standard purification. It is possible achieve a good separation in a single step by HPLC, after a simple extraction with acidic water.
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