Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/3772
Title: Micro-determination of amino acid composition of proteins electroblotted onto polyvinylidene difluoride membranes
Authors: Santucci, Annalisa 
Lozzi, Luisa
Bracci, Luisa
Petreni, S.
Rustici, M.
Soldani, Patrizia 
Neri, Paolo
Issue Date: 1989
Project: None 
Journal: ITALIAN JOURNAL OF BIOCHEMISTRY
Abstract: 
A method for determination of amino acid composition of proteins separated by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes is described. A single blotted band containing 50 to 200 pmoles of protein was cut out and submitted to acid hydrolysis with HCl followed by derivatization with phenylisothiocyanate. The amino acid derivatives were separated by reverse phase high-performance liquid chromatography. Bovine serum albumin, lysozyme, myoglobin, ovalbumin, soybean trypsin inhibitor and carbonic anhydrase were analyzed; the results revealed a good correspondence with reported values. This can be considered an analytical method to determine the amino acid composition of samples from microquantities of protein mixtures, particularly in those cases in which SDS-polyacrylamide gel electrophoresis is the most suitable separation system.
Description: 
36055
URI: http://hdl.handle.net/20.500.12779/3772
ISSN: 0021-2938
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