Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/3733
Title: High performance liquid chromatography immunoaffinity purification of antibodies and antibody fragments
Authors: Santucci, Annalisa 
Soldani, Patrizia 
Lozzi, Luisa
Rustici, M
Bracci, Luisa
Petreni, S
Neri, Paolo
Issue Date: 1988
Project: None 
Journal: JOURNAL OF IMMUNOLOGICAL METHODS
Abstract: 
A rapid antibody purification method is described which combines high performance liquid chromatography (HPLC) resolution with affinity chromatography specificity. The antigen used as immobilized ligand is bound to the HPLC column matrix by reacting the amino groups of the protein with the active epoxy groups of the latter. Once the reaction has finished, the unreacted groups of the column are saturated with an appropriate scavenger. Unrelated proteins are then washed out and the specific antibodies recovered by lowering the pH of the elution buffer.
Description: 
24547
URI: http://hdl.handle.net/20.500.12779/3733
ISSN: 0022-1759
DOI: 10.1016/0022-1759(88)90171-8
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